Identification of Renibacterium salmoninarum surface proteins by radioiodination
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چکیده
منابع مشابه
Identification and characterization of Vibrio cholerae surface proteins by radioiodination.
Whole cells and isolated outer membrane from Vibrio cholerae (Classical, Inaba) were radiolabeled with Iodogen or Iodo-beads as catalyst. Radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. Surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine w...
متن کاملFurther characterization of Renibacterium salmoninarum extracellular products.
Renibacterium salmoninarum, the agent of bacterial kidney disease in salmonids, releases high concentrations of extracellular protein in tissues of infected fish. The extracellular protein consists almost entirely of a 57-kDa protein and derivatives of degradation and aggregation of the same molecule. The 57-kDa protein and its derivatives were fractionated into defined ranges of molecular mass...
متن کاملFlavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida
Intra-ovum infection of artificialy contaminated salmonid (rainbow trout or amago salmon) eggs was studied using three fish pathogens (Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida). Artificial contamination was performed using egg-surface contamination or immersion-water contamination. For F. psychrophilum and R. salmoninarum, infection occurred by the entr...
متن کاملGenetic Variation of Renibacterium salmoninarum Genes in Infected Salmonids
I understand that my project will become part of the permanent collection of Oregon State University, Bioresource Research. My signature below authorizes release of my project to any reader upon request. Abstract Renibacterium salmoninarum is the causative agent of bacterial kidney disease in both wild and farmed salmonid species worldwide. The genome of this pathogen has significant synteny to...
متن کاملMolecular differentiation of Renibacterium salmoninarum isolates from worldwide locations.
Renibacterium salmoninarum is a genospecies that is an obligate pathogen of salmonid fish and is capable of intracellular survival. Conventional typing systems have failed to differentiate isolates of R. salmoninarum. We used two methods to assess the extent of molecular variation which was present in isolates from different geographic locations. In one analysis we investigated possible polymor...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1994
ISSN: 0378-1097
DOI: 10.1016/0378-1097(94)90307-7